Claudia Köhler's Lab

Publications

2017

Jiang H, Moreno-Romero J, Santos-González J, De Jaeger G, Gevaert K, Van De Slijke E, Köhler C (2017) Ectopic application of the repressive histone modification H3K9me2 establishes postzygotic reproductive isolation in Arabidopsis thaliana. Genes and Dev., in press.

Cabout S, Leask MP, Varghese S, Yi J, Peters B, Liu Conze L, Köhler C, Brownfield L (2017) The Meiotic Regulator JASON Utilizes Alternative Translation Initiation Sites to Produce Differentially Localized Forms. Exp. Bot. in press.

Trejo-Arellano MS, Mahrez W, Nakamura M, Moreno-Romero J, Nanni P, Köhler C, Hennig L. (2017) H3K23me1 is an evolutionary conserved histone modification associated with CG DNA methylation in Arabidopsis. Plant J., 90:293-303.(PubMed)

Lafon-Placette C, Johannessen IM, Hornslien KS, Ali MF, Bjerkan KN, Bramsiepe J, Glöckle BM, Rebernig CA, Brysting AK, Grini PE, Köhler C. (2017) Endosperm-based hybridization barriers explain the pattern of gene flow between Arabidopsis lyrata and Arabidopsis arenosa in Central Europe. PNAS, 114: E1027-E1035. (PubMed)

Wang G, Köhler C (2017) Epigenetic processes in flowering plant reproduction. J Exp Bot., 68:797-807. (PubMed)

Martinez G, Köhler, C. (2017) Role of small RNAs in epigenetic reprogramming during plant sexual reproduction. Curr Opin Plant Biol 36: 22–28. (PubMed)

Moreno-Romero J, Santos-Gonzàlez J, Hennig L, Köhler C. (2017) Applying the INTACT method to purify endosperm nuclei and to generate parental-specific epigenome profiles. Nature Protocols, 12:238-254. (PubMed)

2016

Figueiredo DD, Batista RA, Roszak PJ, Hennig, L, Köhler C. (2016) Auxin production in the endosperm drives seed coat development in Arabidopsis. eLIFE, e20542. (PubMed)

Castellano M, Martinez G, Marques MC, Moreno-Romero J, Köhler C, Pallas V, Gomez G. (2016) Changes in the DNA methylation pattern of the host male gametophyte of viroid-infected cucumber plants. J Exp Bot. 67:5857-5868. (PubMed)

Derkacheva M, Liu S, Figueiredo DD, Gentry M, Mozgova I, Nanni P, Tang M, Mannervik M, Köhler C, Hennig L. (2016) H2A deubiquitinases UBP12/13 are part of the Arabidopsis polycomb group protein system Nat Plants. 2:16126 (PubMed)

Hatorangan MR, Laenen B, Steige K, Slotte T, Köhler C. (2016) Rapid Evolution of Genomic Imprinting in Two Species of the Brassicaceae. Plant Cell. 28:1815-27. (PubMed)

Mozgova I, Köhler C. (2016) DNA-sequence-specific erasers of epigenetic memory.  Nat Genet. 48(6):591-2. (PubMed)

Moreno-Romero J, Jiang H, Santos-González J, Köhler C (2016) Parental epigenetic asymmetry of PRC2-mediated histone modifications in the Arabidopsis endosperm. EMBO J. e201593534. (PubMed)

Mahrez W, Shin J, Muñoz-Viana R, Figueiredo DD, Trejo-Arellano MS, Exner V, Siretskiy A, Gruissem W, Köhler C, Hennig L. (2016) BRR2a Affects Flowering Time via FLC Splicing. PLoS Genet. 21;12(4):e1005924. (PubMed)

Martínez G,  Panda K, Köhler C, Slotkin RK (2016) Silencing in sperm cells is directed by RNA movement from the surrounding nurse cell.  Nature Plants 2: 16030 (link)

See also News and Views

Lafon-Placette C, Köhler C. (2016) Endosperm-based postzygotic hybridization barriers: developmental mechanisms and evolutionary drivers.Mol Ecol. 2016 Jan 28. in press (PubMed)

Mahrez W, Arellano MS, Moreno-Romero J, Nakamura M, Shu H, Nanni P, Köhler C, Gruissem W, Hennig L. (2016) H3K36ac Is an Evolutionary Conserved Plant Histone Modification That Marks Active Genes. Plant Physiol. 2016 Mar;170(3):1566-77. (PubMed)

Lafon-Placette C, Vallejo-Marín M, Parisod C, Abbott RJ, Köhler C. (2016) Current plant speciation research: unravelling the processes and mechanisms behind the evolution of reproductive isolation barriers. New Phytol. 209(1):29-33. (PubMed)

Duarte D Figueiredo, Claudia Köhler (2016) Bridging the generation gap: communication between maternal sporophyte, female gametophyte and fertilization products. Curr Opin in Plant Biol. 29:16–20. (PubMed)

2015

Mozgová I, Köhler C, Gaudin V, Hennig L. (2015) The many faces of plant chromatin: Meeting summary of the 4th European workshop on plant chromatin 2015, Uppsala, Sweden. Epigenetics. 10(11):1084-90. (PubMed)

Figueiredo DD, Batista RA, Roszak PJ, Köhler C (2015) Auxin production couples endosperm development to fertilization. Nature Plants: 15184 (link)

See also News and Views

Jiang H, Yi J, Boavida LC, Chen Y, Becker JD, Köhler C, McCormick S.(2015)  Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm. Proc Natl Acad Sci U S A. 112:13378-83 (PubMed)

Wolff P, Jiang H, Wang G, Santos-Gonzàlez J, Köhler C (2015) Paternally expressed imprinted genes establish postzygotic hybridization barriers in Arabidopsis thaliana. eLife1 0.7554 (PubMed)

Rebernig CA, Lafon-Placette C, Hatorangan MR, Slotte T, Köhler C (2015) Non-reciprocal Interspecies Hybridization Barriers in the Capsella Genus are Established in the Endosperm. PLoS Genet. 11(6).(PubMed)

Köhler C, Lafon-Placette C (2015) Evolution and function of epigenetic processes in the endosperm. Front Plant Sci. 6:130. (PubMed)

Mozgova I, Köhler C, Hennig L (2015) Keeping the gate closed: functions of the polycomb repressive complex PRC2 in development. Plant J. 2015 Jul; 83(1):121-32.(PubMed)

Brownfield L, Yi J, Jiang H, Minina EA, Twell D, Köhler C (2015) Organelles maintain spindle position in plant meiosis. Nature Comm. 6: 6492. (PubMed)

Schoft VK, Chumak N, Bindics J, Slusarz L, Twell D, Köhler C, Tamaru H. (2015) SYBR Green-activated sorting of Arabidopsis pollen nuclei based on different DNA/RNA content. Plant Reprod. 28: 61-72. (PubMed)

Lafon-Placette, C., Köhler, C. (2015) Epigenetic mechanisms of postzygotic reproductive isolation in plants. Curr. Opin. Plant Biol., 23C:39-44. (PubMed)

2014

Schatlowski N, Wolff P, Santos-González J, Schoft V, Siretskiy A, Scott R, Tamaru H, Köhler C (2014) Hypomethylated Pollen Bypasses the Interploidy Hybridization Barrier in Arabidopsis. Plant Cell,26:3556-68. (Medline)

See editorial comment on our work here

Rodríguez-Sanz, H., Moreno-Romero, J., Solís, M.T., Köhler, C., Risueño, M.C., Testillano, P.S. (2014) Changes in histone methylation and acetylation during microspore reprogramming to embryogenesis occur concomitantly with Bn HKMT and Bn HAT expression and are associated with cell totipotency, proliferation, and differentiation in Brassica napus. Cytogenet Genome Res 143:209-18. (Medline)

Figueiredo DD, Köhler C. (2014) Signalling events regulating seed coat development. Biochem Soc Trans. 42:358-63. (Medline)

Lafon-Placette C, Köhler C. (2014) Embryo and endosperm, partners in seed development. Curr Opin Plant Biol. 17: 64-9. (Medline)

Jarillo JA, Gaudin V, Hennig L, Köhler C, Piñeiro M. (2014) Plant chromatin warms up in Madrid: Meeting summary of the 3rd European Workshop on Plant Chromatin 2013, Madrid, Spain. Epigenetics 9: (Medline)

Weinhofer I, Köhler C. (2014) Endosperm-specific chromatin profiling by fluorescence-activated nuclei sorting and ChIP-on-chip. Methods Mol Biol. 1112:105-15. (Medline)

2013

Kradolfer D, Wolff P, Jiang H, Siretskiy A, Köhler C. (2013) An imprinted gene underlies postzygotic reproductive isolation in Arabidopsis thaliana. Dev Cell. 26: 525-35. (Medline)

Kradolfer D, Hennig H, Köhler C (2013) Increased maternal genome dosage bypasses the requirement of the FIS Polycomb Repressive Complex 2 in Arabidopsis seed development. PLoS Genet. 9: e1003163. (Medline)

2012

Schatlowski N, Köhler C (2012) Tearing down barriers: understanding the molecular mechanisms of interploidy hybridizations Journal of Experimental Botany, 63: 6059-6067 (Medline)

Jiang H, Köhler C (2012) Evolution, function, and regulation of genomic imprinting in plant seed development (2012) Journal of Experimental Botany 63: 4712-4722 (Medline)

Hehenberger E, Kradolfer D, Köhler C (2012) Endosperm cellularization defines an important developmental transition for embryo development. Development, 139: 2031-203 (Medline)

Houba-Hérin N, Hennig L, Köhler C, Gaudin V (2012) A fruitful chromatin harvest: meeting summary of the Second European Workshop on Plant Chromatin 2011 in Versailles, France. Epigenetics, 7: 307-311. (Medline)

Köhler C, Wolff P, Spillane C (2012) Epigenetic mechanisms underlying genomic imprinting in plants. Annu Rev Plant Biol., 63: 331-352. (Medline )

2011

Roszak P, Köhler C (2011) Polycomb group proteins are required to couple seed coat initiation to fertilization (2011) Proceedings of the National Academy of Sciences of the USA  108:20826-31 (Medline)

McKeown PC, Laouielle-Duprat S, Prins P, Wolff P, Schmid MW, Donoghue MT, Fort A, Duszynska D, Comte A, Lao NT, Wennblom TJ, Smant G, Kohler C, Grossniklaus U, Spillane C. (2011) Identification of imprinted genes subject to parent-of-origin specific expression in Arabidopsis thaliana seeds. BMC Plant. Biol.11:113. (Medline )

Wolff P, Weinhofer I, Seguin J, Roszak P, Beisel C, Donoghue MT, Spillane C, Nordborg M, Rehmsmeier M, Köhler C (2011) High-Resolution Analysis of Parent-of-Origin Allelic Expression in the Arabidopsis Endosperm. PLoS Genet. 6:e102126 (Medline)

Köhler C, Kradolfer D. (2011) Epigenetic mechanisms in the endosperm and their consequences for the evolution of flowering plants. Biochim Biophys Acta. 1809: 438-43. (Medline)

Aichinger, E., Villar, C.B., Di Mambro, R., Sabatini, S., Köhler, C. (2011) The CHD3 Chromatin Remodeler PICKLE and Polycomb Group Proteins Antagonistically Regulate Meristem Activity in the Arabidopsis Root. Plant Cell 23: 1047-1060 (Medline)Brownfield, L., Köhler, C. (2010) Unreduced gamete formation in plants: mechanisms and prospects. J Exp Bot. 62:1659-1668 (Medline)

2010

Egli, B., Kölling, K., Köhler, C., Zeeman, S.C., Streb, S. (2010) Cytosolic phosphoglucomutase is required for gametophyte development in Arabidopsis. Plant Physiol. 154:1659-1671(Medline)

Aichinger, E., Köhler, C. (2010) Bisulphite sequencing of plant genomic DNA. Methods Mol Biol. 655: 433-443. (Medline)

Villar, C.B., Köhler, C. (2010) Plant chromatin immunoprecipitation. Methods Mol Biol. 655:401-411. (Medline)

Köhler, C., Hennig, L. (2010) Regulation of cell identity by plant Polycomb and trithorax group proteins. Curr Opin Genet Dev. 20:541-547. (Medline)

Weinhofer, I., Hehenberger, E., Roszak, P., Hennig, L., Köhler, C. (2010) H3K27me3 Profiling of the Endosperm Implies Exclusion of Polycomb group Protein Targeting by DNA Methylation. PLoS Genet 6: e1001152. (Medline)

Köhler, C., Mittelsten Scheid, O., Erilova, A. (2010) The impact of the triploid block on the origin and evolution of polyploid plants. Trends Genet. 26:142-148. (Medline)

Köhler, C., Gaudin, V., Hennig, L. (2010) Green chromatin dynamics in Zurich: Meeting summary based on the European Workshop on Plant Chromatin 2009 in Zurich, Switzerland. Epigenetics 5:80-83 (Medline)

Köhler, C., Aichinger, E. (2010) Antagonizing Polycomb group-mediated gene repression by chromatin remodelers. Epigenetics 5:20.23. (Medline)

Köhler, C., Weinhofer-Molisch, I. (2010) Mechanisms and evolution of genomic imprinting in plants. Heredity 105:57-63. (Medline)

2009

Erilova, E., Brownfield, L., Exner, V., Rosa, M., Twell, D., Mittelsten Scheid, O., Hennig, L., Köhler, C. (2009) Imprinting of the Polycomb Group Gene MEDEA Serves as a Ploidy Sensor in Arabidopsis. PLoS Genet. 5:e1000663. (Medline)

Aichinger, E., Villar, C.B.R., Farrona, S., Reyes, J.C., Hennig, L., Köhler, C. (2009) CHD3 proteins and Polycomb group proteins antagonistically determine cell identity in Arabidopsis. PLoS Genet. (8):e1000605. (Medline)

Exner, V., Aichinger, E., Shu, H., Wildhaber, T., Alfarano, P., Caflisch, A., Gruissem, W., Köhler, C., Hennig, L. (2009) The chromodomain of LIKE HETEROCHROMATIN PROTEIN 1 is essential for H3K27me3 binding and function during Arabidopsis development. PLoS ONE 4:e5335. (Medline)

Villar, B.R., Erilova, A., Makarevich, G., Trösch, R., Köhler, C. (2009) Control of PHERES1 imprinting in Arabidopsis by direct tandem repeats. Mol. Plant, 2:654-660. (Medline)

2008

Köhler, C., Villar, C.B.R. (2008) Programming of gene expression by Polycomb group proteins. Trends Cell Biol. 18:236-243. (Medline)

Makarevich, G., Villar, C.B.R., Erilova, A., Köhler, C. (2008) Mechanism of PHERES1 imprinting in Arabidopsis. J. Cell Science, 121: 906-912. (Medline)

2007

Leroy, O, Hennig, L, Breuninger, H, Laux, T, Köhler, C (2007) Polycomb group proteins function in the female gametophyte to determine seed development in plants. Development 134:3639-3648. (Medline)

Hennig, L, Köhler, C (2007) Case studies for transcriptional profiling. EXS 97:87-97. (Medline)

2006

Köhler, C, Makarevich, G. (2006) Epigenetic mechanisms governing seed development in plants. EMBO Rep. 7:1223-1227. (Medline)

Makarevich, G, Leroy, O, Akinci, U, Schubert, D, Clarenz, O, Goodrich, J, Grossniklaus, U, Köhler, C. (2006) Different Polycomb group complexes regulate common target genes in Arabidopsis. EMBO Rep. 7:947-952. (Medline)

Schönrock N, Bouveret R, Leroy O, Borghi L, Kohler C, Gruissem W, Hennig L. (2006) Polycomb-group proteins repress the floral activator AGL19 in the FLC-independent vernalization pathway. Genes Dev. 20:1667-78. (Medline)

2005

Köhler C, Grossniklaus U. (2005) Seed development and genomic imprinting in plants. Prog Mol Subcell Biol. 38:237-262. (Medline)

Köhler C, Page DR, Gagliardini V, Grossniklaus U. (2005) The Arabidopsis thaliana MEDEA Polycomb group protein controls expression of PHERES1 by parental imprinting. Nat Genet. 37:28-30. (Medline)

2004

Hennig L, Gruissem W, Grossniklaus U, Köhler C. (2004) Transcriptional programs of early reproductive stages in Arabidopsis. Plant Phys., 135:1765-1775. (Medline)

Collinge MA, Spillane C, Köhler C, Gheyselinck J, Grossniklaus U. (2004) Genetic interaction of an origin recognition complex subunit and the Polycomb group gene MEDEA during seed development. Plant Cell. 16(4):1035-46. (Medline)

Page DR, Köhler C, da Costa-Nunes JA, Baroux C, Moore JM, Grossniklaus U. (2004) Intrachromosomal excision of a hybrid Ds element induces large genomic deletions in Arabidopsis. Proc. Natl. Acad. Sci. USA 101(9):2969-74. (Medline)

2003

Köhler C, Hennig L, Bouveret R, Gheyselinck J, Grossniklaus U, Gruissem W. (2003) Arabidopsis MSI1 is a component of the MEA/FIE Polycomb group complex and required for seed development. EMBO J. 22:4804-14. (Medline)

Köhler C, Hennig L, Spillane C, Pien S, Gruissem W, Grossniklaus U. (2003) The Polycomb group protein MEDEA regulates seed development by controlling expression of the MADS-box gene PHERES1. Genes & Dev. 17:1540-53. (Medline)

Balague C, Lin B, Alcon C, Flottes G, Malmstrom S, Köhler C, Neuhaus G, Pelletier G, Gaymard F, Roby D. (2003) HLM1, an essential signaling component in the hypersensitive response, is a member of the cyclic nucleotide-gated ion channel family. Plant Cell 15:365-379. (Medline)

2002

Köhler C, Grossniklaus U. (2002) Epigenetic inheritance of expression states in plant development: the role of Polycomb group proteins. Curr. Opin. Cell Biol. 14:773-779. (Medline)

Köhler C, Grossniklaus U. (2002) Epigenetics: the flowers that come in from the cold. Curr Biol. 12:129-131. (Medline)

2001

Grossniklaus U, Spillane C, Page DR, Köhler C. (2001) Genomic imprinting and seed development: endosperm formation with and without sex. Curr. Opin. Plant Sci. 4:21-27. (Medline)

Köhler C, Merkle T, Roby D, Neuhaus G. (2001) Developmentally regulated expression of a cyclic nucleotide-gated ion channel from Arabidopsis indicates its involvement in programmed cell death. Planta 213:327-332. (Medline)

2000

Spillane C, MacDougall C, Stock C, Köhler C, Vielle-Calzada J-P, Nunes SM, Grossniklaus U, Goodrich J (2000) Interaction of the Arabidopsis Polycomb group proteins FIE and MEA mediates their common phenotypes. Curr. Biol. 10:1535-1538. (Medline)

Köhler C, Neuhaus G. (2000) Characterisation of calmodulin binding to cyclic nucleotide-gated ion channels from Arabidopsis thaliana. FEBS Lett. 471:133-136. (Medline)

1999

Haasen D, Köhler C, Neuhaus G, Merkle T. (1999) Nuclear export of proteins in plants: AtXPO1 is the export receptor for leucine-rich nuclear export signals in Arabidopsis thaliana. Plant J. 20:695-705. (Medline)

Köhler C, Merkle T, Neuhaus G. (1999) Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana. Plant J. 18:97-104. (Medline)

1998

Oono Y, Chen QG, Overvoorde PJ, Köhler C, Theologis A. (1998) Age mutants of Arabidopsis exhibit altered auxin-regulated gene expression. Plant Cell 10: 1649-1662.

Köhler C, Neuhaus G. (1998) Cloning and partial characterization of two putative cyclic nucleotide-regulated ion channels from Arabidopsis thaliana, designated CNGC1 and CNGC2. Plant Physiol. 116: 1604.

1995

Liang X, Oono Y, Shen NF, Köhler C, Li K, Scolnik PA, Theologis A. (1995) Characterization of two members (ACS1 and ACS3) of the 1-aminocyclopropane-1-carboxylatesynthase gene family of Arabidopsis thaliana. Gene 167: 17-24.